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genome wide crispr cas9 screen  (Addgene inc)


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    Addgene inc genome wide crispr cas9 screen
    Genome Wide Crispr Cas9 Screen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome+wide+crispr+cas9+screens/Human+Genome-wide+CRISPRi-v2+Libraries+(Pooled+Libraries+%2383969%2C+%231000000090)/10__21203_slash_rs__3__rs___7691213_slash_v1-173-16-26
    Average 95 stars, based on 95 article reviews
    genome wide crispr cas9 screen - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Genome Wide:

    Article Title: Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines.
    Article Snippet: .. Genome- wide CRISPR-Cas9 screens are increasingly used for identifying receptors, antiviral proteins, and other impor- tant genes in virus–host interaction and virus restriction factors.89,118–120 CRISPR-Cas9 sgRNA libraries can be designed using online tools and databases such as CasOFFinder (www.biootools.com),121 Addgene (https:// www.addgene.org/crispr/libraries/), Cas-Database,122 CRISP-view,123 DepMap,124 BioGRID ORCS,125 and lately iCSDB database (https://www.kobic.re.kr/icsdb/), DepMap portal, and BioGRID ORCS.126 ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia.
    Article Snippet: Cell lines were propagated in RPMI-1640 media 110 supplemented with recombinant mSCF produced by a Chinese hamster ovary cell line, 5 ng/ml 111 mIL3 (Biolegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For 112 the genome-wide CRISPR/Cas9 screens, 48 million cells from each MA-Cas9 and HM-Cas9 113 lines were spinfected with the GeCKO v2 library (Addgene, # 1000000052) in the presence of 8 114 g /mL polybrene (Sigma Aldrich # TR-1003) using freshly prepared virus at a MOI ~0.25, as 115 previously described (14), followed by 72h of selection with 10 μg/ml of puromycin (BioShop). ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia
    Article Snippet: Cell lines were propagated in RPMI-1640 media supplemented with recombinant murine stem cell factor (mSCF) produced by a Chinese hamster ovary cell line, 5 ng/mL murine interleukin 3 (mIL3) (BioLegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For the genome-wide CRISPR/Cas9 screens, 4.8 × 10 7 cells from each MA-Cas9 and HM-Cas9 line were spinfected with the GeCKO v2 library (Addgene, catalog no. 1000000052) in the presence of 8 μg/mL Polybrene (Sigma-Aldrich, catalog no. TR-1003) using freshly prepared virus at a multiplicity of infection of ∼0.25, as previously described, followed by 72 hours of selection with 10 μg/mL of puromycin (BioShop). ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: .. Genome-wide CRISPR-Cas9 screens were performed using the Bassik Human CRISPR Knockout Library (Addgene pooled libraries nos. ..

    CRISPR:

    Article Title: Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines.
    Article Snippet: .. Genome- wide CRISPR-Cas9 screens are increasingly used for identifying receptors, antiviral proteins, and other impor- tant genes in virus–host interaction and virus restriction factors.89,118–120 CRISPR-Cas9 sgRNA libraries can be designed using online tools and databases such as CasOFFinder (www.biootools.com),121 Addgene (https:// www.addgene.org/crispr/libraries/), Cas-Database,122 CRISP-view,123 DepMap,124 BioGRID ORCS,125 and lately iCSDB database (https://www.kobic.re.kr/icsdb/), DepMap portal, and BioGRID ORCS.126 ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia.
    Article Snippet: Cell lines were propagated in RPMI-1640 media 110 supplemented with recombinant mSCF produced by a Chinese hamster ovary cell line, 5 ng/ml 111 mIL3 (Biolegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For 112 the genome-wide CRISPR/Cas9 screens, 48 million cells from each MA-Cas9 and HM-Cas9 113 lines were spinfected with the GeCKO v2 library (Addgene, # 1000000052) in the presence of 8 114 g /mL polybrene (Sigma Aldrich # TR-1003) using freshly prepared virus at a MOI ~0.25, as 115 previously described (14), followed by 72h of selection with 10 μg/ml of puromycin (BioShop). ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia
    Article Snippet: Cell lines were propagated in RPMI-1640 media supplemented with recombinant murine stem cell factor (mSCF) produced by a Chinese hamster ovary cell line, 5 ng/mL murine interleukin 3 (mIL3) (BioLegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For the genome-wide CRISPR/Cas9 screens, 4.8 × 10 7 cells from each MA-Cas9 and HM-Cas9 line were spinfected with the GeCKO v2 library (Addgene, catalog no. 1000000052) in the presence of 8 μg/mL Polybrene (Sigma-Aldrich, catalog no. TR-1003) using freshly prepared virus at a multiplicity of infection of ∼0.25, as previously described, followed by 72 hours of selection with 10 μg/mL of puromycin (BioShop). ..

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: .. Genome-wide CRISPR-Cas9 screens were performed using the Bassik Human CRISPR Knockout Library (Addgene pooled libraries nos. ..

    Virus:

    Article Title: Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines.
    Article Snippet: .. Genome- wide CRISPR-Cas9 screens are increasingly used for identifying receptors, antiviral proteins, and other impor- tant genes in virus–host interaction and virus restriction factors.89,118–120 CRISPR-Cas9 sgRNA libraries can be designed using online tools and databases such as CasOFFinder (www.biootools.com),121 Addgene (https:// www.addgene.org/crispr/libraries/), Cas-Database,122 CRISP-view,123 DepMap,124 BioGRID ORCS,125 and lately iCSDB database (https://www.kobic.re.kr/icsdb/), DepMap portal, and BioGRID ORCS.126 ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia.
    Article Snippet: Cell lines were propagated in RPMI-1640 media 110 supplemented with recombinant mSCF produced by a Chinese hamster ovary cell line, 5 ng/ml 111 mIL3 (Biolegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For 112 the genome-wide CRISPR/Cas9 screens, 48 million cells from each MA-Cas9 and HM-Cas9 113 lines were spinfected with the GeCKO v2 library (Addgene, # 1000000052) in the presence of 8 114 g /mL polybrene (Sigma Aldrich # TR-1003) using freshly prepared virus at a MOI ~0.25, as 115 previously described (14), followed by 72h of selection with 10 μg/ml of puromycin (BioShop). ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia
    Article Snippet: Cell lines were propagated in RPMI-1640 media supplemented with recombinant murine stem cell factor (mSCF) produced by a Chinese hamster ovary cell line, 5 ng/mL murine interleukin 3 (mIL3) (BioLegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For the genome-wide CRISPR/Cas9 screens, 4.8 × 10 7 cells from each MA-Cas9 and HM-Cas9 line were spinfected with the GeCKO v2 library (Addgene, catalog no. 1000000052) in the presence of 8 μg/mL Polybrene (Sigma-Aldrich, catalog no. TR-1003) using freshly prepared virus at a multiplicity of infection of ∼0.25, as previously described, followed by 72 hours of selection with 10 μg/mL of puromycin (BioShop). ..

    Selection:

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia.
    Article Snippet: Cell lines were propagated in RPMI-1640 media 110 supplemented with recombinant mSCF produced by a Chinese hamster ovary cell line, 5 ng/ml 111 mIL3 (Biolegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For 112 the genome-wide CRISPR/Cas9 screens, 48 million cells from each MA-Cas9 and HM-Cas9 113 lines were spinfected with the GeCKO v2 library (Addgene, # 1000000052) in the presence of 8 114 g /mL polybrene (Sigma Aldrich # TR-1003) using freshly prepared virus at a MOI ~0.25, as 115 previously described (14), followed by 72h of selection with 10 μg/ml of puromycin (BioShop). ..

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia
    Article Snippet: Cell lines were propagated in RPMI-1640 media supplemented with recombinant murine stem cell factor (mSCF) produced by a Chinese hamster ovary cell line, 5 ng/mL murine interleukin 3 (mIL3) (BioLegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For the genome-wide CRISPR/Cas9 screens, 4.8 × 10 7 cells from each MA-Cas9 and HM-Cas9 line were spinfected with the GeCKO v2 library (Addgene, catalog no. 1000000052) in the presence of 8 μg/mL Polybrene (Sigma-Aldrich, catalog no. TR-1003) using freshly prepared virus at a multiplicity of infection of ∼0.25, as previously described, followed by 72 hours of selection with 10 μg/mL of puromycin (BioShop). ..

    Infection:

    Article Title: The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia
    Article Snippet: Cell lines were propagated in RPMI-1640 media supplemented with recombinant murine stem cell factor (mSCF) produced by a Chinese hamster ovary cell line, 5 ng/mL murine interleukin 3 (mIL3) (BioLegend), 10% fetal bovine serum (Wisent), and penicillin/streptomycin (Lonza). .. For the genome-wide CRISPR/Cas9 screens, 4.8 × 10 7 cells from each MA-Cas9 and HM-Cas9 line were spinfected with the GeCKO v2 library (Addgene, catalog no. 1000000052) in the presence of 8 μg/mL Polybrene (Sigma-Aldrich, catalog no. TR-1003) using freshly prepared virus at a multiplicity of infection of ∼0.25, as previously described, followed by 72 hours of selection with 10 μg/mL of puromycin (BioShop). ..

    Knock-Out:

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis
    Article Snippet: .. Genome-wide CRISPR-Cas9 screens were performed using the Bassik Human CRISPR Knockout Library (Addgene pooled libraries nos. ..



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